A fast neutron deletion mutagenesis-based reverse genetics system for plants
نویسندگان
چکیده
منابع مشابه
A fast neutron deletion mutagenesis-based reverse genetics system for plants.
A new reverse genetics method has been developed to identify and isolate deletion mutants for targeted plant genes. Deletion mutant libraries are generated using fast neutron bombardment. DNA samples extracted from the deletion libraries are used to screen for deletion mutants by polymerase chain reaction (PCR) using specific primers flanking the targeted genes. By adjusting PCR conditions to p...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2001
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.2001.01084.x